GPR142 抗体 (Extracellular)
Quick Overview for GPR142 抗体 (Extracellular) (ABIN7237652)
抗原
See all GPR142 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 291-304, Extracellular
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原理
- A Rabbit Polyclonal antibody to GPR142 (extracellular)
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特异性
- Extracellular, 3rd loop.
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,human - identical
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产品特性
- Extracellular, 3rd loop
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142
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亚型
- IgG
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anti-G Protein-Coupled Receptor 142 (GPR142) (Internal Region) antibody
GPR142 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) antibodyGPR142 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (AA 1-50) antibodyGPR142 适用: 人 WB, ELISA, IF, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (Internal Region) antibodyVerified GPR142 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (AA 1-50) antibodyGPR142 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (AA 86-114), (N-Term) antibodyGPR142 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB28809 unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (AA 93-122), (N-Term) antibodyGPR142 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000
Application Dilutions Western blot wb: 1:400
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说明
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Negative Control: (ABIN7235372)
Blocking Peptide: (ABIN7235372)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR142 (G Protein-Coupled Receptor 142 (GPR142))
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别名
- GPR142
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背景
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G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2,G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs1.The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33 % amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 - 1 mM2. GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management3. Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown4.
Alternative names: G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2 -
基因ID
- 217302
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NCBI登录号
- NM_181790
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UniProt
- Q7TQN9
抗原
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