G Protein-Coupled Receptor 132 抗体 (Extracellular, Loop 2)
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Quick Overview for G Protein-Coupled Receptor 132 抗体 (Extracellular, Loop 2) (ABIN7237639)
抗原
See all G Protein-Coupled Receptor 132 (GPR132) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 184-198, Extracellular, Loop 2
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原理
- A Rabbit Polyclonal antibody to GPR132 (extracellular)
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat - 11 out of 15 amino acid residues identical It won't recognize human GPR132
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产品特性
- Extracellular, 2nd loop
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- CFEPLRMNSKIAGYH, corresponding to amino acid residues 184 - 198 of mouse GPR132
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亚型
- IgG
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anti-G Protein-Coupled Receptor 132 (GPR132) (C-Term) antibody
GPR132 适用: 人, 小鼠, 猴 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (C-Term) antibodyGPR132 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (Extracellular Domain) antibodyGPR132 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (2nd Extracellular Loop), (AA 181-195) antibody (FITC)GPR132 适用: 人 FACS, LCI 宿主: 兔 Polyclonal FITC
anti-G Protein-Coupled Receptor 132 (GPR132) (C-Term) antibodyGPR132 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (C-Term), (Cytoplasmic Domain) antibodyGPR132 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (2nd Extracellular Loop), (AA 181-195) antibodyGPR132 适用: 人 WB, FACS, LCI 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: 2.5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235365)
Blocking Peptide: (ABIN7235365)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- G Protein-Coupled Receptor 132 (GPR132)
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别名
- GPR132
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背景
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Synonyms: G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A
Description: G-protein coupled receptor 132, (GPR132, G2A) is classified as a member of the proton sensing G-protein coupled receptor (GPCR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is expressed on the cell surface membrane and contains seven transmembrane domains, similar to serpentine receptors. GPR132 senses changes in extracellular pH , and activation of this proton-sensing receptor by acidosis transduces multiple downstream G protein signaling pathways1.GPR132 is expressed in a broad range of immunoregulatory cell types, including macrophages, dendritic cells, neutrophils, mast cells, T and B lymphocytes. It mediates immune cell migration as well as phagocytosis and regulates autoimmunity and lymphocyte homeostasis2.G2A also plays a role in tumor suppression: the tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion and hence, G2A and other proton sensing receptors may be involved in regulation of cancer cell metastasis and proliferation. Small molecule modulators of the pH -sensing GPCRs are being actively developed and evaluated. Research on pH -sensing GPCRs provides important insights into the molecular interaction between the tumor and its acidic microenvironment and may identify new targets for cancer therapy3.
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基因ID
- 56696
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NCBI登录号
- NM_013345
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UniProt
- Q9Z282
抗原
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