G Protein-Coupled Receptor 132 抗体 (Extracellular)
Quick Overview for G Protein-Coupled Receptor 132 抗体 (Extracellular) (ABIN7237639)
抗原
See all G Protein-Coupled Receptor 132 (GPR132) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 184-198, Extracellular
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原理
- A Rabbit Polyclonal antibody to GPR132 (extracellular)
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特异性
- Extracellular, 2nd loop.
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat - 11 out of 15 amino acid residues identical It won't recognize human GPR132
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产品特性
- Extracellular, 2nd loop
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: GST fusion protein,
Immunogen Sequence: Peptide CFEPLRMNSKIAGYH, corresponding to amino acid residues 184 - 198 of mouse GPR132
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亚型
- IgG
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anti-G Protein-Coupled Receptor 132 (GPR132) (C-Term) antibody
GPR132 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (Extracellular Domain) antibodyGPR132 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (C-Term) antibodyGPR132 适用: 人, 小鼠, 猴 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (C-Term) antibodyGPR132 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (C-Term), (Cytoplasmic Domain) antibodyGPR132 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 132 (GPR132) (AA 181-195), (Extracellular) antibodyGPR132 适用: 人 WB, FACS, LCI 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235365)
Blocking Peptide: (ABIN7235365)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- G Protein-Coupled Receptor 132 (GPR132)
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别名
- GPR132
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背景
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G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A,G-protein coupled receptor 132, (GPR132, G2A) is classified as a member of the proton sensing G-protein coupled receptor (GPCR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is expressed on the cell surface membrane and contains seven transmembrane domains, similar to serpentine receptors. GPR132 senses changes in extracellular pH , and activation of this proton-sensing receptor by acidosis transduces multiple downstream G protein signaling pathways1.GPR132 is expressed in a broad range of immunoregulatory cell types, including macrophages, dendritic cells, neutrophils, mast cells, T and B lymphocytes. It mediates immune cell migration as well as phagocytosis and regulates autoimmunity and lymphocyte homeostasis2.G2A also plays a role in tumor suppression: the tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion and hence, G2A and other proton sensing receptors may be involved in regulation of cancer cell metastasis and proliferation. Small molecule modulators of the pH -sensing GPCRs are being actively developed and evaluated. Research on pH -sensing GPCRs provides important insights into the molecular interaction between the tumor and its acidic microenvironment and may identify new targets for cancer therapy3.
Alternative names: G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A -
基因ID
- 56696
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NCBI登录号
- NM_013345
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UniProt
- Q9Z282
抗原
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