CLEC7A 抗体 (Extracellular)
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Quick Overview for CLEC7A 抗体 (Extracellular) (ABIN7237603)
抗原
See all CLEC7A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 87-102, Extracellular
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原理
- A Rabbit Polyclonal antibody to CLEC7A/Dectin-1 (extracellular)
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特异性
- Extracellular, C-terminus.
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat - 15 out of 16 amino acid residues identical Not recommended for human samples
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产品特性
- Extracellular, C-terminus
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RNKENHKPTESSLDEK, corresponding to amino acid residues 87 - 102 of mouse CLEC7A
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亚型
- IgG
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anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) (AA 66-247) antibody
CLEC7A 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 4G2G6 unconjugated
anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) (AA 17-244) antibodyCLEC7A 适用: 小鼠 FACS, WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) (AA 41-140) antibodyCLEC7A 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) (AA 1-44) antibodyCLEC7A 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) antibodyCLEC7A 适用: 人 FACS 宿主: 小鼠 Monoclonal BD6 unconjugated
anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) antibody (PE)CLEC7A 适用: 人 FACS 宿主: 小鼠 Monoclonal 15E2 PE
anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) antibody (APC)CLEC7A 适用: 人 FACS 宿主: 小鼠 Monoclonal 15E2 APC
anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) antibodyCLEC7A 适用: 人 FACS, IHC (fro) 宿主: 小鼠 Monoclonal 15E2 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600-1:2400
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7234892)
Blocking Peptide: (ABIN7234892)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- CLEC7A (C-Type Lectin Domain Family 7, Member A (CLEC7A))
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别名
- CLEC7A
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背景
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C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369,Dectin-1, encoded by CLEC7A, is a small transmembrane receptor that is a member of the C-type lectin family of pattern-recognition receptors (PRRs)1,2. As this receptor specifically recognizes and binds to β-glucans polysaccharides derived from the cell walls of fungi and yeast, it has been characterized for its role in antifungal immunity3.Dectin-1 is predominately expressed on macrophages, dendritic cells, and neutrophils4,5. Upon ligation of β-glucan, Dectin-1 recruits the adaptor protein CARD9 to phosphorylate spleen tyrosine kinase (Syk) which in turn initiates a signaling cascade which induces phagocytosis and leads to the activation of nuclear factor-κB (NF-κB) and MAPK6. The Dectin-1 Syk mediated pathway has been demonstrated to be activated and involved in the alternative activation of macrophages as Dectin-1 was upregulated in vitro within 4 hours when stimulated with IL-4 and IL-13, cytokines which induce alternative activation of macrophages7. Moreover, the activated Syk pathway has also been implicated in renal interstitial fibrotic disease where it was demonstrated that suppressing Syk in an experimental mouse model of renal fibrosis inhibits the progression of kidney fibrosis, shown by a reduction in mRNA expression of Arginase-1 (murine M2 macrophage marker)8.While the Dectin-1 pathway has been shown to be involved in the bleomycin-induced model of skin fibrosis, Dectin-1 signaling and receptor expression has yet to be investigated in the context of pulmonary fibrosis9.
Alternative names: C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369 -
基因ID
- 56644
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NCBI登录号
- NM_001128431
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UniProt
- Q6QLQ4
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途径
- Activation of Innate immune Response
抗原
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