TGM3 抗体
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Quick Overview for TGM3 抗体 (ABIN7233203)
抗原
See all TGM3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Transglutaminase-3 (TG3) Mouse Monoclonal Antibody
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特异性
- This antibody recognizes full-length human TG3 (77 kDa) and the proteolysed form of human TG3 (30-47 kDa).
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交叉反应
- 人
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免疫原
- Recombinant human TG3 (accession no. Q08188).
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亚型
- IgG1
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anti-Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) (AA 601-693) antibody
TGM3 适用: 小鼠, 大鼠 ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) (AA 468-693) antibodyTGM3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) (AA 1-180) antibodyTGM3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) antibodyTGM3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) antibodyTGM3 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) (AA 468-693) antibodyTGM3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) (AA 598-610) antibodyVerified TGM3 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) (N-Term) antibodyTGM3 适用: 人, 小鼠, 大鼠, 犬, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3) antibodyTGM3 适用: 人 IHC, ELISA, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoblotting: use at 1-5 μg/mL. Bands of ~77 kDa and ~30 kDa are detected.
Immunocytochemistry: use at 2-10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 μg/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
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缓冲液
- 0.1M Tris, 0.1M glycine, 2 % sucrose
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储存条件
- -20 °C,-80 °C
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储存方法
- This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
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- TGM3 (Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3))
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别名
- TGM3
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背景
- Protein-glutamine γ-glutamyltransferase E,Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. {ECO:0000250}.,Transglutaminase-3 (TG3), an epidermal- type enzyme, is responsible for formation of the epidermis. During keratinocyte differentiation, TG1 and TG3 appear to act cooperatively in the cross-linking of proteins, including involucrin, loricrin and small proline-rich proteins (SPRs). These reactions result in formation of the cornified envelope (CE), a specialized component consisting of covalent cross- links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Furthermore, TG3 in hair follicles is involved in cross-linking structural proteins such as trichohyalin and keratin intermediate to hardening the inner root sheath. During differentiation in these processes, a zymogen form of TG3 (77 kDa) is activated by limited proteolysis with cathepsins S and/or L.,Cytoplasm,EC 2.3.2.13, Transglutaminase E, TG(E, TGE, TGase E, Transglutaminase-3, TGase-3 [Cleaved into: Protein-glutamine γ-glutamyltransferase E 50 kDa catalytic chain, Protein-glutamine γ-glutamyltransferase E 27 kDa non-catalytic chain]
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UniProt
- Q08188
抗原
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