CENPA 抗体
Our Local Distributor
北京 101111
Quick Overview for CENPA 抗体 (ABIN7232821)
抗原
See all CENPA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-CENP-A Mouse Monoclonal Antibody
-
特异性
- This antibody recognizes human CENP-A. Reactivity with other species has not been investigated.
-
交叉反应
- 人
-
免疫原
- Synthetic peptide corresponding to a sequence within human CENP-A.
-
亚型
- IgG1
-
-
anti-Centromere Protein A (CENPA) (pSer16), (pSer18) antibody
CENPA 适用: 人 WB, IF, ICC, DB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein A (CENPA) (N-Term) antibodyCENPA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein A (CENPA) (AA 1-100) antibodyCENPA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein A (CENPA) antibodyKD Validated CENPA 适用: 人 WB 宿主: 兔 Monoclonal 25GB95 unconjugated Recombinant Antibody
anti-Centromere Protein A (CENPA) antibodyCENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 unconjugated
anti-Centromere Protein A (CENPA) antibody (HRP)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 HRP
anti-Centromere Protein A (CENPA) antibody (FITC)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 FITC
anti-Centromere Protein A (CENPA) antibody (Biotin)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 Biotin
anti-Centromere Protein A (CENPA) antibody (PE)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 PE
anti-Centromere Protein A (CENPA) antibody (PerCP)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 PerCP
-
-
应用备注
-
Immunoblotting: use at 1 μg/mL. A band of ~18 kDa is detected.Detection of CENP-A in lysate of U2OS cellsELISA: use at 1-10 μg/mL with CENP-A on the solid phase.
Positive control: U2OS cell lysate
These are recommended concentrations,
Enduser should determine optimal concentrations for their applications. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
溶解方式
- Dilute in PBS or medium that is identical to that used in the assay system.
-
浓度
- 1.0 mg/mL
-
缓冲液
- PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- This antibody is stable for at least one (1) year at -20°C.
-
-
- CENPA (Centromere Protein A (CENPA))
-
别名
- CENPA
-
背景
- Histone H3-like centromeric protein A,Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292). {PubMed:11756469, PubMed:14667408, PubMed:15282608, PubMed:15475964, PubMed:15702419, PubMed:17651496, PubMed:18072184, PubMed:19114591, PubMed:21478274, PubMed:23818633, PubMed:25556658, PubMed:26878239, PubMed:27499292, PubMed:7962047, PubMed:9024683, PubMed:20739937}.,Replicated chromosomes include two kinetochores that control chromosome segregation during mitosis. Centromere Protein-A (CENP-A), a histone H3-like protein, contains a C-terminal H3-like domain which is required for centromere localization of CENP-A. It is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H2A, H2B, and H4. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on chromosomes.,Nucleus, Chromosome, centromere, kinetochore, Chromosome, centromere,Centromere autoantigen A, Centromere protein A, CENP-A
-
NCBI登录号
- NP_001035891
-
UniProt
- P49450
-
途径
- Chromatin Binding, Maintenance of Protein Location
抗原
-
