HYOU1 抗体
Quick Overview for HYOU1 抗体 (ABIN7232796)
抗原
See all HYOU1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Grp170 Mouse Monoclonal Antibody
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特异性
- This antibody recognizes human, mouse, and rat Grp170 (170 kDa).
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified by Protein G affinity chromatography
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免疫原
- Synthetic peptide sequence derived from human Grp170 (accession no. NP_006389.3)
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亚型
- IgG2b
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anti-Hypoxia Up Regulated 1 (HYOU1) (C-Term) antibody
HYOU1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Up Regulated 1 (HYOU1) (AA 901-999) antibodyHYOU1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 6F7 unconjugated
anti-Hypoxia Up Regulated 1 (HYOU1) (AA 401-500) antibodyHYOU1 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Up Regulated 1 (HYOU1) (AA 695-994) antibodyHYOU1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Up Regulated 1 (HYOU1) antibodyHYOU1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 6E3-2C3 unconjugated
anti-Hypoxia Up Regulated 1 (HYOU1) antibody (HRP)HYOU1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 6E3-2C3 HRP
anti-Hypoxia Up Regulated 1 (HYOU1) antibody (FITC)HYOU1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 6E3-2C3 FITC
anti-Hypoxia Up Regulated 1 (HYOU1) antibody (Biotin)HYOU1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 6E3-2C3 Biotin
anti-Hypoxia Up Regulated 1 (HYOU1) antibody (PE)HYOU1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 6E3-2C3 PE
anti-Hypoxia Up Regulated 1 (HYOU1) antibody (APC)HYOU1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 6E3-2C3 APC
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应用备注
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ELISA: confirmed with antibody (1-10 μg/mL) binding to Grp170 at 5 μg/mL on the solid phase
Immunoblotting: with antibody at 1.0-2.0 μg/mL. A band of 170 kDa is detected.
These are recommended concentrations
End user should determine optimal concentrations for their applications.
Positive control: HEK293 cell lysate. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Dilute in PBS or medium that is identical to that used in the assay system.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide, 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- This antibody is stable for at least one (1) year at -20°C.
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- HYOU1 (Hypoxia Up Regulated 1 (HYOU1))
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别名
- HYOU1
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背景
- Hypoxia up-regulated protein 1,Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. {PubMed:10037731}.,Grp170 is a major chaperone/stress protein resident in the ER. It is distantly related in sequence to both hsp110 and hsp70 families, but represents the Grp170 stress protein family. Studies have suggested that Grp170 is an important element of the protein processing machinery of the ER. Unlike Hsp110 and Hsp70, Grp170 has two distinct chaperoning domains, a classic beta-sheet peptide binding domain, with some COOH- terminal sequence, and a COOH-terminal alpha-helical domain. Like other members of the Hsp70 superfamily, Grp170 can elicit anti-tumor immune responses most likely by chaperoning antigenic peptide and binding to receptor(s) on antigen-presenting cells (APC).,Endoplasmic reticulum lumen.,150 kDa oxygen-regulated protein, ORP-150, 170 kDa glucose-regulated protein, GRP-170
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NCBI登录号
- NP_006389
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UniProt
- Q9Y4L1
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途径
- ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome
抗原
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