WT1 抗体 (AA 310-390)
Quick Overview for WT1 抗体 (AA 310-390) (ABIN7232174)
抗原
See all WT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 310-390
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原理
- WT1 Polyclonal Antibody
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特异性
- The antibody detects endogenous levels of WT1 protein
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纯化方法
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen
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免疫原
- Synthesized peptide derived from part region of human WT1 protein at AA range: 310-390
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- WT1 (Wilms Tumor 1 (WT1))
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别名
- WT1
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背景
- Rabbit Anti-WT1 Polyclonal Antibody,WT33,WT1 encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. WT1 exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID: 7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.,WT1
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分子量
- observerd band 49kDa
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基因ID
- 7490
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UniProt
- P19544
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途径
- Tube Formation
抗原
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