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WT1 抗体 (AA 310-390)

This anti-WT1 antibody is a 兔 多克隆 antibody detecting WT1 in WB 和 ELISA. Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN7232174
发货至: 中国

Quick Overview for WT1 抗体 (AA 310-390) (ABIN7232174)

抗原

See all WT1 抗体
WT1 (Wilms Tumor 1 (WT1))

适用

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人, 大鼠, 小鼠

宿主

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克隆类型

  • 106
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多克隆

标记

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This WT1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
  • 抗原表位

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    AA 310-390

    原理

    WT1 Polyclonal Antibody

    特异性

    The antibody detects endogenous levels of WT1 protein

    纯化方法

    The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

    免疫原

    Synthesized peptide derived from part region of human WT1 protein at AA range: 310-390

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

    说明

    Primary Antibody

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • 抗原

    WT1 (Wilms Tumor 1 (WT1))

    别名

    WT1

    背景

    Rabbit Anti-WT1 Polyclonal Antibody,WT33,WT1 encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. WT1 exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID: 7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.,WT1

    分子量

    observerd band 49kDa

    基因ID

    7490

    UniProt

    P19544

    途径

    Tube Formation
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