V-ATPase D1 抗体 (Internal Region)
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北京 101111
Quick Overview for V-ATPase D1 抗体 (Internal Region) (ABIN7217604)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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原理
- V-ATPase D1 Polyclonal Antibody
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特异性
- V-ATPase D1 Polyclonal Antibody detects endogenous levels of V-ATPase D1 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the Internal region of human V-ATPase D1
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- V-ATPase D1 (VHA36-1) (Vacuolar H[+] ATPase Subunit 36-1 (VHA36-1))
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别名
- V-ATPase D1
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背景
- Rabbit Anti-V-ATPase D1 Polyclonal Antibody,ATP6V0D1, ATP6D, VPATPD, V-type proton ATPase subunit d 1, V-ATPase subunit d 1, 32 kDa accessory protein, V-ATPase 40 kDa accessory protein, V-ATPase AC39 subunit, p39, Vacuolar proton pump subunit d 1,ATP6V0D1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.,V-type proton ATPase subunit d 1
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分子量
- observerd band 40kDa
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基因ID
- 9114
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UniProt
- P61421
抗原
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