UBE3B 抗体 (AA 550-630)
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Quick Overview for UBE3B 抗体 (AA 550-630) (ABIN7225756)
抗原
See all UBE3B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 550-630
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原理
- UBE3B Polyclonal Antibody
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特异性
- UBE3B Polyclonal Antibody detects endogenous levels of UBE3B protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the Internal region of human UBE3B at AA range: 550-630
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亚型
- IgG
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anti-Ubiquitin Protein Ligase E3B (UBE3B) (C-Term) antibody
UBE3B 适用: 人 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3B (UBE3B) (AA 720-769) antibodyUBE3B 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, Bat, 小鸡, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3B (UBE3B) (Middle Region) antibodyUBE3B 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3B (UBE3B) (Middle Region) antibodyUBE3B 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3B (UBE3B) (AA 581-630) antibodyUBE3B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3B (UBE3B) antibodyUBE3B 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))
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别名
- UBE3B
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背景
- Rabbit Anti-UBE3B Polyclonal Antibody,UBE3B, Ubiquitin-protein ligase E3B,The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: e1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. UBE3B (ubiquitin protein ligase E3B) encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for UBE3B.,Ubiquitin-protein ligase E3B
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分子量
- observerd band 123kDa
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基因ID
- 89910
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UniProt
- Q7Z3V4
抗原
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