TNNI2 抗体 (pThr142)
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Quick Overview for TNNI2 抗体 (pThr142) (ABIN7224726)
抗原
See all TNNI2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr142
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原理
- Troponin I-C (phospho Thr142) Polyclonal Antibody
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特异性
- Phospho-Troponin I-C (T142) Polyclonal Antibody detects endogenous levels of Troponin I-C protein only when phosphorylated at T142.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Troponin I-C Phospho-Thr142
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- TNNI2 (Fast Skeletal Troponin I (TNNI2))
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别名
- Troponin I-C
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背景
- Rabbit Anti-Troponin I-C (phospho Thr142) Polyclonal Antibody,TNNI3, TNNC1, Troponin I, cardiac muscle, Cardiac troponin I,Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. NNI3 (troponin I3, cardiac type) encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in TNNI3 cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).,Troponin I cardiac muscle
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分子量
- observerd band 28kDa
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基因ID
- 7137
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UniProt
- P19429
抗原
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