SHB 抗体 (pTyr246)
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北京 101111
Quick Overview for SHB 抗体 (pTyr246) (ABIN7213118)
抗原
See all SHB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr246
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原理
- Shb (phospho Tyr246) Polyclonal Antibody
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特异性
- Phospho-Shb (Y246) Polyclonal Antibody detects endogenous levels of Shb protein only when phosphorylated at Y246.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Shb Phospho-Tyr246
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亚型
- IgG
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anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) (AA 348-446) antibody
SHB 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4C11 unconjugated
anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) (AA 250-290) antibodySHB 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB47168 unconjugated
anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) (C-Term) antibodySHB 适用: 人, 小鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) (pTyr246) antibodySHB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) (C-Term) antibodySHB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) antibodySHB 适用: 人 WB 宿主: 兔 Monoclonal 24GB2010 unconjugated Recombinant Antibody
anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) (N-Term) antibodySHB 适用: 人, 小鼠, 大鼠, Cow, 兔, 犬, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) (pTyr246) antibodySHB 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) (AA 35-84) antibodySHB 适用: 人, 小鼠, 大鼠, Cow, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Src Homology 2 Domain Containing Adaptor Protein B (SHB) (N-Term) antibodySHB 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- SHB (Src Homology 2 Domain Containing Adaptor Protein B (SHB))
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别名
- Shb
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背景
- Rabbit Anti-Shb (phospho Tyr246) Polyclonal Antibody,SHB, SH2 domain-containing adapter protein B,SHB (SH2 homology protein in B/ beta -cells) is a 55-59 kDa cytoplasmic adaptor protein that serves as a link between phosphotyrosine residues and downstream signaling pathways. SHB is ubiquitously expressed, and binds tyrosine kinase receptors such as FGFR1, VEGFR2, and the TCR (zeta -chain) following their activation. It is highly modular, and through a variety of motifs, is able to bind multiple, structurally unrelated proteins that collectively generate a signal transduction network. Human SHB is 509 amino acids (aa) in length. It contains an N-terminal Pro-rich region that binds SH3 domain-containing proteins (aa 19-45), a central PTB domain that binds select aa motifs, and a C-terminal SH2 domain that interacts with phosphotyrosines (aa 410-504). There are at least nine utilized phosphorylation sites. There are two isoform variants. One is 66-68 kDa in size, and possesses an 87 aa Pro-rich N-terminal extension. This MW may increase to 77-80 kDa following posttranslational processing. A second variant shows an 18 aa substitution for aa 280-509. Over aa 2-128, human SHB shares 91 % aa identity with mouse SHB.,SH2 domain-containing adapter protein B
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分子量
- observerd band 55kDa
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基因ID
- 6461
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UniProt
- Q15464
抗原
-
