PPP1R12A 抗体 (pThr853)
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北京 101111
Quick Overview for PPP1R12A 抗体 (pThr853) (ABIN7222632)
抗原
See all PPP1R12A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr853
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原理
- MYPT1 (phospho Thr853) Polyclonal Antibody
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特异性
- Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human MYPT1 Phospho-Thr853
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亚型
- IgG
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anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 1-200) antibody
PPP1R12A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibodyVerified PPP1R12A 适用: 人 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 1-40), (N-Term) antibodyPPP1R12A 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibodyPPP1R12A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr696) antibodyPPP1R12A 适用: 人, 大鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibodyPPP1R12A 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 710-970) antibodyPPP1R12A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 481-514), (Ser507) antibodyPPP1R12A 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB56872 unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibodyPPP1R12A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pSer696) antibodyPPP1R12A 适用: 人, 小鼠, 大鼠, 斑马鱼, 小鸡, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
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别名
- MYPT1
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背景
- Rabbit Anti-MYPT1 (phospho Thr853) Polyclonal Antibody,PPP1R12A, MBS, MYPT1, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit,Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP) -bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.,Protein phosphatase 1 regulatory subunit 12A
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分子量
- observerd band 130kDa
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基因ID
- 4659
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UniProt
- O14974
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途径
- M Phase
抗原
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