HLA-DQ1/DQ2 抗体 (Internal Region)
This 兔 多克隆 anti-HLA-DQ1/DQ2 antibody specifically detects HLA-DQ1/DQ2 in ELISA, WB 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN7218502
发货至:
中国
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Quick Overview for HLA-DQ1/DQ2 抗体 (Internal Region) (ABIN7218502)
抗原
HLA-DQ1/DQ2
(MHC Class II HLA-DQ1/DQ2 (HLA-DQ1/DQ2))
适用
人
宿主
兔
克隆类型
多克隆
标记
This HLA-DQ1/DQ2 antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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抗原表位
- Internal Region
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原理
- HLA-DQB1/2 Polyclonal Antibody
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特异性
- HLA-DQB1/2 Polyclonal Antibody detects endogenous levels of HLA-DQB1/2 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the Internal region of human HLA-DQB1/2
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亚型
- IgG
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anti-MHC Class II HLA-DQ1/DQ2 (HLA-DQ1/DQ2) (Internal Region) antibody
HLA-DQ1/DQ2 适用: 人 ELISA, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- HLA-DQ1/DQ2 (MHC Class II HLA-DQ1/DQ2 (HLA-DQ1/DQ2))
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别名
- HLA-DQB1/2
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背景
- Rabbit Anti-HLA-DQB1/2 Polyclonal Antibody,HLA-DQB1, HLA-DQB, HLA class II histocompatibility antigen, DQ beta 1 chain, MHC class II antigen DQB1, HLA-DQB2, HLA-DXB, HLA class II histocompatibility antigen, DQ beta 2 chainHLA class II histocompatibility antigen, DX beta chain, MHC class II antigen DQB2,HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: b lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]HLA-DQB1 (Major Histocompatibility Complex, Class II, DQ Beta 1) is a Protein Coding gene. Diseases associated with HLA-DQB1 include celiac disease and multiple sclerosis, disease progression, modifier of. Among its related pathways are Immune System and Interferon gamma signaling. Gene Ontology (GO) annotations related to this gene include peptide antigen binding and MHC class II receptor activity. An important paralog of this gene is HLA-DMA. Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.,HLA class II histocompatibility antigen DQ beta 1 chain
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分子量
- observerd band 30kDa
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基因ID
- 100507714
抗原
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