MED27 抗体 (AA 90-170)
Quick Overview for MED27 抗体 (AA 90-170) (ABIN7225992)
抗原
See all MED27 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 90-170
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原理
- CRSP34 Polyclonal Antibody
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特异性
- CRSP34 Polyclonal Antibody detects endogenous levels of CRSP34 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the Internal region of human CRSP34 at AA range: 90-170
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- MED27 (Mediator Complex Subunit 27 (MED27))
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别名
- CRSP34
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背景
- Rabbit Anti-CRSP34 Polyclonal Antibody,MED27, CRSP34, CRSP8, Mediator of RNA polymerase II transcription subunit 27, Cofactor required for Sp1 transcriptional activation subunit 8, CRSP complex subunit 8, Mediator complex subunit 27, P37 TRAP/SMCC/PC2 subunit, Transcriptional co,The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED27 (mediator complex subunit 27) is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for MED27, and a pseudogene of MED27 is located on the long arm of chromosome 5.,Mediator of RNA polymerase II transcription subunit 27
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分子量
- observerd band 35kDa
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基因ID
- 9442
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UniProt
- Q6P2C8
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途径
- Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
抗原
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