MED1 抗体 (pThr1457)
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Quick Overview for MED1 抗体 (pThr1457) (ABIN7223308)
抗原
See all MED1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr1457
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原理
- TRAP220 (phospho Thr1457) Polyclonal Antibody
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特异性
- Phospho-TRAP220 (T1457) Polyclonal Antibody detects endogenous levels of TRAP220 protein only when phosphorylated at T1457.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human TRAP220 Phospho-Thr1457
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亚型
- IgG
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anti-Mediator Complex Subunit 1 (MED1) (pThr1457) antibody
MED1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibodyMED1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 1391-1490) antibodyMED1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2A2 unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 1-212) antibodyMED1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 878-1031) antibodyMED1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibodyMED1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (Center) antibodyMED1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (pThr1457) antibodyMED1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (Internal Region) antibodyMED1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (Thr2647) antibodyMED1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- MED1 (Mediator Complex Subunit 1 (MED1))
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别名
- TRAP220
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背景
- Rabbit Anti-TRAP220 (phospho Thr1457) Polyclonal Antibody,MED1, ARC205, CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2, Mediator of RNA polymerase II transcription subunit 1, Activator-recruited cofactor 205 kDa component, ARC205, Mediator complex subunit 1, Peroxiso,The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED1 (mediator complex subunit 1) is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. Thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize.,Mediator of RNA polymerase II transcription subunit 1
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分子量
- observerd band 168kDa
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基因ID
- 5469
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UniProt
- Q15648
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途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
抗原
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