KSR1 抗体
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Quick Overview for KSR1 抗体 (ABIN7225680)
抗原
See all KSR1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Ksr-1 Polyclonal Antibody
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特异性
- Ksr-1 Polyclonal Antibody detects endogenous levels of Ksr-1 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Ksr-1 around the non-phosphorylation site of S392
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亚型
- IgG
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anti-Kinase Suppressor of Ras 1 (KSR1) antibody
KD Validated KSR1 适用: 人 WB, FACS, ICC 宿主: 兔 Monoclonal 23GB6290 unconjugated Recombinant Antibody
anti-Kinase Suppressor of Ras 1 (KSR1) (AA 320-420) antibodyKSR1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Kinase Suppressor of Ras 1 (KSR1) (AA 404-598) antibodyKSR1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Kinase Suppressor of Ras 1 (KSR1) (AA 301-400) antibodyKSR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6C7 unconjugated
anti-Kinase Suppressor of Ras 1 (KSR1) (AA 358-407) antibodyKSR1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Kinase Suppressor of Ras 1 (KSR1) (AA 601-750) antibodyKSR1 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Kinase Suppressor of Ras 1 (KSR1) (pSer392) antibodyKSR1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Kinase Suppressor of Ras 1 (KSR1) (AA 301-400) antibodyKSR1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Kinase Suppressor of Ras 1 (KSR1) antibodyKSR1 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Kinase Suppressor of Ras 1 (KSR1) (pSer392) antibodyKSR1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- KSR1 (Kinase Suppressor of Ras 1 (KSR1))
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别名
- Ksr-1
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背景
- Rabbit Anti-Ksr-1 Polyclonal Antibody,KSR1, KSR, Kinase suppressor of Ras 1,KSR1 (kinase supressor of Ras) was identified from a genetic screen in Drosophila and C. elegans as a component of the Ras signaling pathway. KSR1 has a putative carboxy-terminal kinase domain that lacks a key Lys residue for phospho-group transfer. Although reports indicate that ceramide and EGF activate KSR1, other evidence demonstrates that KSR1 regulates Raf in a kinase-independent manner. It is now widely accepted that KSR1 functions as a scaffold that binds MEK1/2 and 14-3-3 protein constitutively and binds ERK1/2 in a Ras activation-dependent manner. HSP70/HSP90 and p50 Cdc37 associate with the KSR1 complex to ensure its stability. Multiple phosphorylation sites have been identified: ser297 and Ser392 mediate 14-3-3 binding, and putative MAPK phosphorylation sites include Thr260, Thr274 and Ser443. C-TAK1 (Cdc25C-associated kinase 1) binds and phosphorylates KSR1 at Ser392 in quiescent cells. In response to stimuli, Ser392 is dephosphorylated by PP2A, which leads to ERK1/2 association and allows the KSR1 complex to translocate from cytosol to membrane, where the MAPK pathway is activated. IMP, a Ras-responsive E3 ubiquitin ligase, is also involved in interaction with KSR1 and may regulate its localization and stability. Very high expression levels of KSR1 inhibit MAPK signaling, whereas physiological levels promote MAPK signaling, indicating that the scaffold protein can turn signaling "on" or "off" depending on the scaffold concentration.,Kinase suppressor of Ras 1
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分子量
- observerd band 115kDa
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基因ID
- 8844
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UniProt
- Q8IVT5
抗原
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