GUF1 抗体 (AA 390-470)
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Quick Overview for GUF1 抗体 (AA 390-470) (ABIN7224038)
抗原
See all GUF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 390-470
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原理
- GUF1 Polyclonal Antibody
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特异性
- GUF1 Polyclonal Antibody detects endogenous levels of GUF1 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the Internal region of human GUF1 at AA range: 390-470
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亚型
- IgG
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anti-GUF1 GTPase Homolog (GUF1) (AA 183-212), (N-Term) antibody
GUF1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32635 unconjugated
anti-GUF1 GTPase Homolog (GUF1) antibodyGUF1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GUF1 GTPase Homolog (GUF1) (AA 496-545) antibodyGUF1 适用: 人, 小鼠, Cow, 犬, 豚鼠, 斑马鱼, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-GUF1 GTPase Homolog (GUF1) (C-Term) antibodyGUF1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-GUF1 GTPase Homolog (GUF1) (AA 190-220), (N-Term) antibodyGUF1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- GUF1 (GUF1 GTPase Homolog (GUF1))
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别名
- GUF1
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背景
- Rabbit Anti-GUF1 Polyclonal Antibody,GUF1, Translation factor GUF1, mitochondrial, Elongation factor 4 homolog, EF-4, GTPase GUF1, Ribosomal back-translocase,GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.,Translation factor GUF1 mitochondrial
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基因ID
- 60558
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UniProt
- Q8N442
抗原
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