EIF5B 抗体 (AA 1020-1100)
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Quick Overview for EIF5B 抗体 (AA 1020-1100) (ABIN7226064)
抗原
See all EIF5B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1020-1100
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原理
- eIF5B Polyclonal Antibody
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特异性
- EIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the C-terminal region of human eIF5B at AA range: 1020-1100
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))
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别名
- eIF5B
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背景
- Rabbit Anti-eIF5B Polyclonal Antibody,EIF5B, IF2, KIAA0741, Eukaryotic translation initiation factor 5B, eIF-5B, Translation initiation factor IF-2,Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B (eukaryotic translation initiation factor 5B). The gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.,Eukaryotic translation initiation factor 5B
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分子量
- observerd band 139kDa
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基因ID
- 9669
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UniProt
- O60841
抗原
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