EIF4E 抗体 (pSer209)
Quick Overview for EIF4E 抗体 (pSer209) (ABIN7212948)
抗原
See all EIF4E 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer209
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原理
- eIF4E (phospho Ser209) Polyclonal Antibody
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特异性
- Phospho-eIF4E (S209) Polyclonal Antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human eIF4E Phospho-Ser209
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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别名
- eIF4E
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背景
- Rabbit Anti-eIF4E (phospho Ser209) Polyclonal Antibody,EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein,Eukaryotic translation initiation factor 4E encoded by EIF4E is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs.Eukaryotic translation initiation factor 4E aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. EIF4E acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of EIF4E are found on other chromosomes. Alternative splicing results in multiple transcript variants.,Eukaryotic translation initiation factor 4E
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分子量
- observerd band 25kDa
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基因ID
- 1977
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UniProt
- P06730
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途径
- BCR Signaling
抗原
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