EIF4B 抗体 (pSer422)
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北京 101111
Quick Overview for EIF4B 抗体 (pSer422) (ABIN7221930)
抗原
See all EIF4B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer422
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原理
- eIF4B (phospho Ser422) Polyclonal Antibody
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特异性
- Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human eIF4B Phospho-Ser422
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody
EIF4B 适用: 人, 小鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibodyEIF4B 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibodyEIF4B 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (AA 352-611) antibodyEIF4B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (AA 546-575), (C-Term) antibodyEIF4B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB11717 unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (C-Term) antibodyEIF4B 适用: 人, 小鼠, 大鼠, 马, Cow, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (AA 353-382) antibodyEIF4B 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibodyEIF4B 适用: 人, 小鼠 WB, IF, EIA 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (AA 362-590) antibodyEIF4B 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) (pSer422) antibodyEIF4B 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))
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别名
- eIF4B
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背景
- Rabbit Anti-eIF4B (phospho Ser422) Polyclonal Antibody,EIF4B, Eukaryotic translation initiation factor 4B, eIF-4B,Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens , and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.,Eukaryotic translation initiation factor 4B
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分子量
- observerd band 80kDa
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基因ID
- 3716
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UniProt
- P23588
抗原
-
