EIF4G1 抗体
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Quick Overview for EIF4G1 抗体 (ABIN7220206)
抗原
See all EIF4G1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- eIF4G Polyclonal Antibody
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特异性
- EIF4G Polyclonal Antibody detects endogenous levels of eIF4G protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human eIF4G around the non-phosphorylation site of S1148
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))
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别名
- eIF4G
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背景
- Rabbit Anti-eIF4G Polyclonal Antibody,EIF4G1, EIF4F, EIF4G, EIF4GI, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220,The eukaryotic translation initiation factor 4 gamma 1 encoded by EIF4G1 is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.,Eukaryotic translation initiation factor 4 gamma 1
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分子量
- observerd band 175kDa
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基因ID
- 1981
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UniProt
- Q04637
抗原
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