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E2F4 抗体

This 兔 多克隆 anti-E2F4 antibody specifically detects E2F4 in WB, ELISA 和 IHC (p). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7218178
发货至: 中国
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Quick Overview for E2F4 抗体 (ABIN7218178)

抗原

See all E2F4 抗体
E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

适用

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人, 小鼠, 大鼠

宿主

  • 51
  • 5
  • 2

克隆类型

  • 40
  • 18
多克隆

标记

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This E2F4 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 原理

    E2F-4 Polyclonal Antibody

    特异性

    E2F-4 Polyclonal Antibody detects endogenous levels of E2F-4 protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    免疫原

    Synthesized peptide derived from human E2F-4 around the non-acetylation site of K96

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

    说明

    Primary Antibody

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • 抗原

    E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

    别名

    E2F-4

    背景

    Rabbit Anti-E2F-4 Polyclonal Antibody,E2F4, Transcription factor E2F4, E2F-4,Transcription factor E2F4 encoded by E2F4 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.,Transcription factor E2F4

    分子量

    observerd band 43kDa

    基因ID

    1874

    UniProt

    Q16254

    途径

    Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
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