E2F2 抗体 (AA 190-270)
Quick Overview for E2F2 抗体 (AA 190-270) (ABIN7214646)
抗原
See all E2F2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 190-270
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原理
- E2F-2 Polyclonal Antibody
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特异性
- E2F-2 Polyclonal Antibody detects endogenous levels of E2F-2 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the Internal region of human E2F-2 at AA range: 190-270
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- E2F2 (E2F Transcription Factor 2 (E2F2))
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别名
- E2F-2
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背景
- Rabbit Anti-E2F-2 Polyclonal Antibody,E2F2, Transcription factor E2F2, E2F-2,E2F transcription factor 2 encoded by E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1.,Transcription factor E2F2
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分子量
- observerd band 48kDa
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基因ID
- 1870
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UniProt
- Q14209
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication
抗原
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