DAB1 抗体 (pTyr232)
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Quick Overview for DAB1 抗体 (pTyr232) (ABIN7225606)
抗原
See all DAB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr232
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原理
- Dab1 (phospho Tyr232) Polyclonal Antibody
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特异性
- Phospho-Dab1 (Y232) Polyclonal Antibody detects endogenous levels of Dab1 protein only when phosphorylated at Y232.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Dab1 Phospho-Tyr232
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亚型
- IgG
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anti-Disabled Homolog 1 (Drosophila) (DAB1) (pTyr232) antibody
DAB1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 400-555) antibodyDAB1 适用: 小鼠 WB, ELISA, IHC, IP, FM 宿主: 兔 Polyclonal unconjugated
anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 400-555) antibodyDAB1 适用: 小鼠 WB, ELISA, IHC, IP, FM 宿主: 兔 Polyclonal unconjugated
anti-Disabled Homolog 1 (Drosophila) (DAB1) (pTyr232) antibodyDAB1 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 1-185) antibodyDAB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Disabled Homolog 1 (Drosophila) (DAB1) (Ser296) antibodyDAB1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Disabled Homolog 1 (Drosophila) (DAB1) (pTyr198) antibodyDAB1 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 1-553) antibodyDAB1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 187-236) antibodyDAB1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Disabled Homolog 1 (Drosophila) (DAB1) (C-Term) antibodyDAB1 适用: 人, 大鼠, 小鼠, Pig, 猴, Cow, 犬, 马, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))
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别名
- Dab1
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背景
- Rabbit Anti-Dab1 (phospho Tyr232) Polyclonal Antibody,DAB1, Disabled homolog 1,The Reelin signaling pathway plays a critical role in neuronal development. Reelin is a secreted glycoprotein that binds to the lipoprotein receptors VLDLR and ApoER2 or alpha3beta1 integrin on the surface of neurons. Activation of these receptors induces tyrosine phosphorylation of Disabled 1 (Dab1), an intracellular adaptor. It is generally believed that tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is the most critical downstream event in Reelin signaling. The phosphotyrosine-binding (PTB) domain within its amino terminus enables Dab1 to recognize and bind to a conserved sequence motif within the cytoplasmic tail of the receptors. In addition, the PTB contains a Pleckstrin Homology-like subdomain that binds to phosphoinositides. The phosphoinositide-binding region within the Dab1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab1 independent of VLDLR and ApoER2. It has been demonstrated that Src, CrkII, CrkL and Dock1 associate with tyrosine-phosphorylated Dab. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232.,Disabled homolog 1
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分子量
- observerd band 60kDa
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基因ID
- 8674
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UniProt
- O75553
抗原
-
