DBF4 抗体 (AA 10-90)
Quick Overview for DBF4 抗体 (AA 10-90) (ABIN7214522)
抗原
See all DBF4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 10-90
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原理
- Dbf4 Polyclonal Antibody
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特异性
- Dbf4 Polyclonal Antibody detects endogenous levels of Dbf4 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the N-terminal region of human Dbf4 at AA range: 10-90
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亚型
- IgG
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anti-DBF4 Homolog (DBF4) (AA 2-98) antibody
DBF4 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6G9 unconjugated
anti-DBF4 Homolog (DBF4) (AA 101-281) antibodyDBF4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) antibodyDBF4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB5370 unconjugated Recombinant Antibody
anti-DBF4 Homolog (DBF4) antibodyDBF4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3815 unconjugated Recombinant Antibody
anti-DBF4 Homolog (DBF4) (AA 2-98) antibodyDBF4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (Internal Region) antibodyDBF4 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (N-Term) antibodyDBF4 适用: 人, Cow, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (N-Term) antibodyDBF4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (Internal Region) antibodyVerified DBF4 适用: 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (N-Term) antibodyDBF4 适用: 人, 小鼠, 猴 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- DBF4 (DBF4 Homolog (DBF4))
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别名
- Dbf4
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背景
- Rabbit Anti-Dbf4 Polyclonal Antibody,DBF4, ASK, DBF4A, ZDBF1, Protein DBF4 homolog A, Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1,The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.,Protein DBF4 homolog A
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分子量
- observerd band 77kDa
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基因ID
- 10926
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UniProt
- Q9UBU7
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途径
- Mitotic G1-G1/S Phases, DNA Replication
抗原
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