Cyclin H 抗体 (pThr315)
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Quick Overview for Cyclin H 抗体 (pThr315) (ABIN7225768)
抗原
See all Cyclin H (CCNH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr315
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原理
- Cyclin H (phospho Thr315) Polyclonal Antibody
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特异性
- Phospho-Cyclin H (T315) Polyclonal Antibody detects endogenous levels of Cyclin H protein only when phosphorylated at T315.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Cyclin H Phospho-Thr315
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- Cyclin H (CCNH)
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别名
- Cyclin H
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背景
- Rabbit Anti-Cyclin H (phospho Thr315) Polyclonal Antibody,CCNH, Cyclin-H, MO15-associated protein, p34, p37,The protein encoded by CCNH (cyclin H)belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of CCNH is found on chromosome 4. Alternate splicing results in multiple transcript variants.,Cyclin-H
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分子量
- observerd band 34kDa
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基因ID
- 902
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UniProt
- P51946
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
抗原
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