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Cyclin B1 抗体 (pSer147)

The 兔 多克隆 anti-Cyclin B1 antibody (ABIN7225714) specifically detects Cyclin B1 in WB, ELISA 和 IHC (p). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7225714
发货至: 中国
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Quick Overview for Cyclin B1 抗体 (pSer147) (ABIN7225714)

抗原

See all Cyclin B1 (CCNB1) 抗体
Cyclin B1 (CCNB1)

适用

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人, 小鼠, 大鼠

宿主

  • 195
  • 41
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克隆类型

  • 146
  • 91
多克隆

标记

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This Cyclin B1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    pSer147

    原理

    Cyclin B1 (phospho Ser147) Polyclonal Antibody

    特异性

    Phospho-Cyclin B1 (S147) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S147.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    免疫原

    Synthesized peptide derived from human Cyclin B1 Phospho-Ser147

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

    说明

    Primary Antibody

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • 抗原

    Cyclin B1 (CCNB1)

    别名

    Cyclin B1

    背景

    Rabbit Anti-Cyclin B1 (phospho Ser147) Polyclonal Antibody,CCNB1, CCNB, G2/mitotic-specific cyclin-B1,The protein encoded by CCNB1 (cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.,cyclin-B1

    分子量

    observerd band 60kDa

    基因ID

    891

    UniProt

    P14635

    途径

    Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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