Cyclin B1 抗体 (pSer147)
Quick Overview for Cyclin B1 抗体 (pSer147) (ABIN7225714)
抗原
See all Cyclin B1 (CCNB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer147
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原理
- Cyclin B1 (phospho Ser147) Polyclonal Antibody
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特异性
- Phospho-Cyclin B1 (S147) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S147.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Cyclin B1 Phospho-Ser147
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- Cyclin B1 (CCNB1)
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别名
- Cyclin B1
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背景
- Rabbit Anti-Cyclin B1 (phospho Ser147) Polyclonal Antibody,CCNB1, CCNB, G2/mitotic-specific cyclin-B1,The protein encoded by CCNB1 (cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.,cyclin-B1
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分子量
- observerd band 60kDa
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基因ID
- 891
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UniProt
- P14635
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途径
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
抗原
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