CDCA3 抗体 (AA 190-270)
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Quick Overview for CDCA3 抗体 (AA 190-270) (ABIN7214118)
抗原
See all CDCA3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 190-270
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原理
- CdcA3 Polyclonal Antibody
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特异性
- CdcA3 Polyclonal Antibody detects endogenous levels of CdcA3 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the C-terminal region of human CdcA3 at AA range: 190-270
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- CDCA3 (Cell Division Cycle Associated 3 (CDCA3))
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别名
- CdcA3
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背景
- Rabbit Anti-CdcA3 Polyclonal Antibody,CDCA3, C8, GRCC8, TOME1, Cell division cycle-associated protein 3, Gene-rich cluster protein C8, Trigger of mitotic entry protein 1, TOME-1,Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. CdcA3 (Cell division cycle-associated protein 3), also known as TOME1 (Trigger of mitotic entry protein 1) and GRCC8 (Gene-rich cluster protein C8), is a 268 amino acid cytoplasmic protein that is required for entry into mitosis. By participating with E3 ligase complexes, CdcA3 is involved in ubiquination and degradation of the mitosis-inhibitory kinase Wee 1, leading to the dephosphorylation of Cdc2 in the late G2 phase. CdcA3 itself is targeted for destruction by anaphase promoting complex (APC) in the G1 phase of the cell cycle, then allowing for Wee 1 accumulation during interphase.,Cell division cycle-associated protein 3
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分子量
- observerd band 28kDa
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基因ID
- 83461
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UniProt
- Q99618
抗原
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