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CDC27 抗体 (AA 330-410)

The 兔 多克隆 anti-CDC27 antibody is suitable to detect CDC27 in samples from 人, 大鼠 和 小鼠. It has been validated for WB, ELISA, IHC 和 IF.
产品编号 ABIN7214104
发货至: 中国
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Quick Overview for CDC27 抗体 (AA 330-410) (ABIN7214104)

抗原

See all CDC27 抗体
CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

适用

  • 75
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  • 1
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人, 大鼠, 小鼠

宿主

  • 86
  • 5

克隆类型

  • 75
  • 16
多克隆

标记

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  • 5
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This CDC27 antibody is un-conjugated

应用范围

  • 33
  • 27
  • 27
  • 26
  • 13
  • 11
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  • 7
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  • 2
  • 2
  • 1
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

    • 16
    • 15
    • 7
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 330-410

    原理

    Rabbit Anti-Cdc27 Polyclonal Antibody

    特异性

    Cdc27 Polyclonal Antibody detects endogenous levels of Cdc27 protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    免疫原

    Synthesized peptide derived from the Internal region of human Cdc27 at AA range: 330-410

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

    有效期

    12 months
  • 抗原

    CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

    别名

    Cdc27

    背景

    CDC27, ANAPC3, D0S1430E, D17S978E, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, APC3, CDC27 homolog, CDC27Hs, H-NUCThe protein encoded by CDC27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and CDC27 product of Schizosaccharomyces pombe nuc 2. Cell division cycle 27 is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.

    分子量

    92kD

    基因ID

    996

    UniProt

    P30260
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