CAMK1 抗体 (pThr177)
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Quick Overview for CAMK1 抗体 (pThr177) (ABIN7225526)
抗原
See all CAMK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr177
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原理
- Rabbit Anti-CaMKIα (phospho Thr177) Polyclonal Antibody
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特异性
- Phospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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免疫原
- Synthesized peptide derived from human CaMKIalpha Phospho-Thr177
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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有效期
- 12 months
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- CAMK1 (Calcium/calmodulin-Dependent Protein Kinase I (CAMK1))
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别名
- CaMKIalpha
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背景
- CAMK1, Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM-KI, CaM kinase I alpha, CaMKI-alphaCalcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
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分子量
- 41kD
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基因ID
- 8536
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UniProt
- Q14012
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途径
- Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Smooth Muscle Cell Migration
抗原
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