Adducin alpha/beta (pSer713), (pSer726) 抗体
The 兔 多克隆 anti-Adducin alpha/beta antibody is suitable to detect Adducin alpha/beta in samples from 人, 小鼠 和 大鼠. It has been validated for ELISA, IF, IHC 和 WB.
产品编号 ABIN7213298
发货至:
中国
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Quick Overview for Adducin alpha/beta (pSer713), (pSer726) 抗体 (ABIN7213298)
抗原
Adducin alpha/beta
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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抗原表位
- pSer713, pSer726
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原理
- Rabbit Anti-Adducin α/β (phospho Ser726/713) Polyclonal Antibody
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特异性
- Phospho-Adducin α/β (S726/713) Polyclonal Antibody detects endogenous levels of Adducin α/β protein only when phosphorylated at S726/713.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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免疫原
- Synthesized peptide derived from human Adducin alpha/beta Phospho-Ser726/713
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亚型
- IgG
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anti-Adducin alpha/beta (Lys5) antibody
适用: 人, 小鼠, 大鼠 ELISA, IF, IHC, WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Adducin alpha/beta (pSer713), (pSer726) antibody适用: 人, 小鼠, 大鼠 ELISA, IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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有效期
- 12 months
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- Adducin alpha/beta
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背景
- ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha, ADD2, ADDB, Beta-adducin, Erythrocyte adducin subunit betaAdducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca (2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described.
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分子量
- 80kD
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基因ID
- 118, 119
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UniProt
- P35611, P35612
抗原
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