YWHAE 抗体 (AA 180-260)
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Quick Overview for YWHAE 抗体 (AA 180-260) (ABIN7213368)
抗原
See all YWHAE 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 180-260
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原理
- 14-3-3 ε Polyclonal Antibody
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特异性
- 14-3-3 ε Polyclonal Antibody detects endogenous levels of 14-3-3 ε protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the C-terminal region of human 14-3-3 epsilon at AA range: 180-260
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- YWHAE (14-3-3 epsilon (YWHAE))
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别名
- 14-3-3 epsilon
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背景
- Rabbit Anti-14-3-3 ε Polyclonal Antibody,YWHAE, 14-3-3 protein epsilon, 14-3-3E,Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for YWHAE.,14-3-3 protein epsilon
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基因ID
- 7531
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UniProt
- P62258
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途径
- Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
抗原
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