CDH6 抗体 (AA 281-380)
(1 validation)Quick Overview for CDH6 抗体 (AA 281-380) (ABIN715286)
抗原
See all CDH6 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 281-380
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交叉反应
- 人
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预测反应
- Mouse,Rat,Cow,Horse
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human K Cadherin
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亚型
- IgG
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anti-K-Cadherin (CDH6) antibody
CDH6 适用: 人, 大鼠 WB, IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal 2B6 unconjugated
anti-K-Cadherin (CDH6) (AA 513-612) antibodyCDH6 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2F2 unconjugated
anti-K-Cadherin (CDH6) antibodyCDH6 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
anti-K-Cadherin (CDH6) (AA 1-250) antibodyCDH6 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) antibodyCDH6 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) (AA 665-695), (C-Term) antibodyCDH6 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) (AA 1-663) antibodyCDH6 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) (C-Term) antibodyCDH6 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) antibodyCDH6 适用: 人, 大鼠, 小鼠, Cow, 小鸡 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) (AA 61-89), (N-Term) antibodyCDH6 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100 -
限制
- 仅限研究用
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- by
- Flow Cytometry & Cell Separation Facility, Purdue University
- No.
- #029743
- 日期
- 2014.07.01
- 抗原
- Lot Number
- 130823
- Method validated
- Flow Cytometry
- Positive Control
- 786-O cells and [MCF-7 cells] (http://www.nature.com/bjc/journal/v90/n5/fig_tab/6601640f10.html#figure-title)
- Negative Control
- Notes
- As expected, strong signal was observed in the cells stained with anti-K-Cadherin (CDH6) antibody. A small amount of non-specific staining was observed from the isotype and secondary antibody only negative controls compared with unstained cells.
- Primary Antibody
- Antigen: K-Cadherin (CDH6) antibody
- Catalog number: ABIN715286
- Lot number: 130823
- Secondary Antibody
- Antibody: Goat anti-rabbit IgG-Alexa 647
- Full Protocol
- Positive control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
- Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - 1 µg primary antibody - 1 µg isotype control antibody
- Cells were incubated for 30 min on ice.
- Labeled cells were washed twice in PBS containing 0.5% BSA.
- Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
- Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
- Labeled cells were washed twice in PBS containing 0.5% BSA.
- Propidium Iodide (PI) was added to discern live cells from dead cells.
- Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
- Experimental Notes
- - The data displayed is gated on PI negative cells.
生效 #029743 (Flow Cytometry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1. Histogram of 786-O cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).]()
Figure 1. Histogram of 786-O cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).
![Figure 2. Histogram of MCF-7 cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).]()
Figure 2. Histogram of MCF-7 cells stained with anti-K-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).
Full Methods -
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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- CDH6 (K-Cadherin (CDH6))
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别名
- K Cadherin
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背景
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Synonyms: CAD6, KCAD, Cadherin-6, Kidney cadherin, K-cadherin, CDH6
Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.
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基因ID
- 1004
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UniProt
- P55285
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途径
- Cell-Cell Junction Organization
抗原
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