POLA1 抗体 (N-Term)
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Quick Overview for POLA1 抗体 (N-Term) (ABIN7184445)
抗原
See all POLA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- POLA1 Antibody
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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免疫原
- Synthesized peptide derived from N-terminal of Human DNA Polymerase alpha.
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亚型
- IgG
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anti-DNA Polymerase alpha (POLA1) (AA 1363-1462) antibody
POLA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1406-1439) antibodyPOLA1 适用: 小鼠 WB, FACS 宿主: 兔 Polyclonal RB57637 unconjugated
anti-DNA Polymerase alpha (POLA1) (N-Term) antibodyPOLA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (N-Term) antibodyPOLA1 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1240-1462) antibodyPOLA1 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 310-832) antibodyPOLA1 适用: Thermus aquaticus WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1-33) antibodyPOLA1 适用: 人 WB 宿主: 兔 Polyclonal RB57636 unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1363-1462) antibodyPOLA1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-DNA Polymerase alpha (POLA1) (AA 1209-1465) antibodyPOLA1 适用: 小鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- POLA1 (DNA Polymerase alpha (POLA1))
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别名
- POLA1
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背景
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Background: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
Wong S.W., EMBO J. 7:37-47(1988).
Pearson B.E., Mol. Cell. Biol. 11:2081-2095(1991).
Hsi K.-L., Nucleic Acids Res. 18:6231-6237(1990).Aliases: DKFZp686K1672 antibody, DNA polymerase alpha 1 catalytic subunit antibody, DNA polymerase alpha catalytic subunit antibody, DNA polymerase alpha catalytic subunit p180 antibody, DNA polymerase alpha p180 subunit antibody, DPOLA_HUMAN antibody, OTTHUMP00000023089 antibody, OTTHUMP00000023090 antibody, P180 antibody, p180 subunit antibody, POLA antibody, POLA1 antibody, Polymerase (DNA directed), alpha 1, catalytic subunit antibody, Polymerase (DNA-directed), alpha (70kD) antibody
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UniProt
- P09884
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途径
- SARS-CoV-2 Protein Interactome
抗原
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