RAG2 抗体 (AA 296-415) (Biotin)
Quick Overview for RAG2 抗体 (AA 296-415) (Biotin) (ABIN7175271)
抗原
See all RAG2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 296-415
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交叉反应
- 人
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纯化方法
- >95%, Protein G purified
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免疫原
- Recombinant Human V(D)J recombination-activating protein 2 protein (296-415AA)
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亚型
- IgG
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anti-Recombination Activating Gene 2 (RAG2) (Center) antibody
RAG2 适用: 人, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 1-527) antibodyRAG2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 428-527) antibodyRAG2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G8 unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 258-527) antibodyRAG2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 498-527), (C-Term) antibodyRAG2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (N-Term) antibodyRAG2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 1-527) antibodyRAG2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 350-527) antibodyRAG2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4D5 unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 296-415) antibodyRAG2 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) antibodyRAG2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4 -
储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- RAG2 (Recombination Activating Gene 2 (RAG2))
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别名
- RAG2
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背景
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Background: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3\'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5\'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at \'Lys-4\' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).
Aliases: RAG 2 antibody, RAG-2 antibody, RAG2 antibody, RAG2_HUMAN antibody, Recombination activating gene 2 antibody, V(D)J recombination activating protein 2 antibody, V(D)J recombination-activating protein 2 antibody
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UniProt
- P55895
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途径
- Chromatin Binding, Production of Molecular Mediator of Immune Response
抗原
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