RDH12 抗体 (AA 1-316)
(1 reference)
(1 validation)-
- 抗原 See all RDH12 抗体
- RDH12 (Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12))
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抗原表位
- AA 1-316
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RDH12 antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (IHC)
- 交叉反应
- 人
- 纯化方法
- >95%, Protein G purified
- 免疫原
- Recombinant Human Retinol dehydrogenase 12 protein (1-316AA)
- 亚型
- IgG
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anti-Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12) (AA 217-316) antibody
RDH12 适用: 人 ELISA, WB, IF 宿主: 小鼠 Monoclonal 1E11 unconjugated
anti-Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12) (AA 182-316) antibodyRDH12 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12) (AA 1-316) antibodyRDH12 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12) (AA 1-316) antibodyRDH12 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12) (AA 179-228) antibodyRDH12 适用: 人, Pig, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12) (AA 215-264) antibodyRDH12 适用: 人, Pig, Cow, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12) (Middle Region) antibodyRDH12 适用: 人, Pig, 大鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Recommended dilution: IHC:1:20-1:200,
- 限制
- 仅限研究用
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- by
- Palczewski Lab, Center For Translational Vision Research, UC Irvine
- No.
- #104484
- 日期
- 2023.03.23
- 抗原
- RDH12
- Lot Number
- E0629A
- Method validated
- Immunohistochemistry
- Positive Control
Retina cryosection from B6 Albino (B6(Cg)-Tyrc-2J/J) animal
- Negative Control
Retina cryosection from B6 Albino (B6(Cg)-Tyrc-2J/J) animal
No primary antibody
- Notes
Passed. Presence of specific signal in the RPE cell layer was considered as indication of specific immunoreactivity using the RDH12 antibody ABIN7167836.
- Primary Antibody
- ABIN7167836
- Secondary Antibody
- donkey anti-rabbit AF647-conjugated antibody (Abcam, 150075)
- Full Protocol
- Collect eyes from mice and fix with paraformaldehyde 4% (Electron Microscopy Sciences, 15710) in 1x PBS for 30 min at RT.
- Cryoprotection with sucrose series:
- Wash in 10% sucrose in 1x PBS.
- Immerse in 10% sucrose in 1x PBS for 30 min at RT.
- Wash in 20% sucrose in 1x PBS.
- Immerse in 20% sucrose in 1x PBS for 30 min RT.
- Wash in 30% sucrose in 1x PBS.
- 30% sucrose ON at 4°C.
- Embed eyes in OCT compound (Tissue-Tek O.C.T. Compound, 4583).
- Cut retinal sections at a thickness of 12 μm on a cryostat.
- Air dry sections for 15 min at RT, store at -80°C until use.
- Bring sections to RT and rehydrate in 1x PBS for 1 h.
- Incubate sections in blocking buffer (1x PBS, 3% BSA (Sigma-Aldrich, A7030), 3% Donkey serum (Sigma-Aldrich, S30-100ML), 0.1% Triton X-100 (Sigma-Aldrich, X100-500ML)) for 1 h at RT.
- Incubate sections with primary rabbit anti-RDH12 antibody (antibodies-online, ABIN7167836, lot E0629A) diluted 1:50 in blocking buffer ON at RT. Include a no primary antibody negative controls. Additionally, counterstaing with primary mouse anti-CRALBP antibody (Thermo Fisher Scientific, MA1-813).
- Incubate sections with secondary AF647-conjugated donkey anti-rabbit antibody (Abcam, Ab150075) or AF488-conjugated donkey anti-mouse antibody (Thermo Fisher Scientific, A32766) diluted 1:500 in blocking buffer for 1 h at RT.
- Rinse sections once with 1x PBS, 0.1% Triton X-100 for 5 min at RT.
- Incubate sections in 1x DAPI (Thermo Fisher Scientific, 62248) in 1x PBS, 0.1% Triton X-100 for 15 min at RT.
- Rinse sections 3x with 1x PBS, 0.1% Triton X-100 for 5 min at RT.
- Mount sections in VECTASHIELD® HardSet™ Antifade Mounting Medium (Vector Laboratories, H-1400) mounting medium.
- Acquire images with a fluorescence microscope and appropriate filter settings.For the validation purposes Keyence BZ-X800E fluorescence microscope was used with following filters: BZ-X DAPI for DAPI, BZ-X GFP for AF488, BZ-X Cy5 for AF647. Images were taken at 10x and 40x magnification.
- Experimental Notes
Experiment involved validation of the specificity of 4 antibodies against different Rdh proteins: Rdh5 (ABIN7254060), Rdh10 (ABIN7118460), Rdh11 (ABIN966957), and Rdh12 (ABIN7167836). All 4 proteins are important for eye function and highly expressed in neural retina and/or RPE. Validation is based on comparison of each staining with known pattern of expression in the mouse retina. For review highlighting each Rdh localization see PMID20801113.
To aid orientation in the cell layers anti-Cralbp counterstain was included in the staining (Thermo MA1-813). Cralbp (Rlbp1) is highly expressed in RPE and Müller glia cells in mouse retina.
生效 #104484 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Retinal sections from the wild-type (B6 albino) mice immunostained with anti-RDH12 antibody ABIN7167836. DAPI staining shows localization of the inner (INL) and outer (ONL) nuclear layer of the mouse retina. Cralbp (Rlbp1) co-staining was used to visualize RPE and Müller glia cells in the retina. Presence of specific signal in the RPE cell layer confirms specific immunoreactivity.]()
Retinal sections from the wild-type (B6 albino) mice immunostained with anti-RDH12 antibody ABIN7167836. DAPI staining shows localization of the inner (INL) and outer (ONL) nuclear layer of the mouse retina. Cralbp (Rlbp1) co-staining was used to visualize RPE and Müller glia cells in the retina. Presence of specific signal in the RPE cell layer confirms specific immunoreactivity.
Full Methods -
- 状态
- Liquid
- 缓冲液
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Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4 - 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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: "Rapid RGR-dependent visual pigment recycling is mediated by the RPE and specialized Müller glia." in: Cell reports, Vol. 42, Issue 8, pp. 112982, (2023) (PubMed).
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: "Rapid RGR-dependent visual pigment recycling is mediated by the RPE and specialized Müller glia." in: Cell reports, Vol. 42, Issue 8, pp. 112982, (2023) (PubMed).
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- 抗原
- RDH12 (Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12))
- 别名
- RDH12 (RDH12 产品)
- 别名
- wu:fj43a10 antibody, zgc:92430 antibody, A930033N07Rik antibody, LCA13 antibody, LCA3 antibody, RP53 antibody, SDR7C2 antibody, DSSDR2 antibody, retinol dehydrogenase 12 (all-trans/9-cis/11-cis) antibody, retinol dehydrogenase 12 antibody, Retinol dehydrogenase 12 antibody, RDH12 antibody, rdh12 antibody, MAV_1968 antibody, CC1G_02720 antibody, Bm1_36660 antibody, PTRG_03574 antibody, PTRG_05326 antibody, PTRG_08067 antibody, LOC100282710 antibody, LOC100285880 antibody, LOC100226769 antibody, Rdh12 antibody
- 背景
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Background: Exhibits an oxidoreductive catalytic activity towards retinoids. Most efficient as an NADPH-dependent retinal reductase. Displays high activity toward 9-cis and all-trans-retinol. Also involved in the metabolism of short-chain aldehydes. No steroid dehydrogenase activity detected. Might be the key enzyme in the formation of 11-cis-retinal from 11-cis-retinol during regeneration of the cone visual pigments.
Aliases: All trans and 9 cis retinol dehydrogenase antibody, All-trans and 9-cis retinol dehydrogenase antibody, LCA 3 antibody, LCA13 antibody, LCA3 antibody, RDH 12 antibody, RDH12 antibody, RDH12_HUMAN antibody, Retinol dehydrogenase 12 (all trans/9 cis/11 cis) antibody, Retinol dehydrogenase 12 all trans and 9 cis antibody, Retinol dehydrogenase 12 antibody, RP53 antibody, SDR7C2 antibody, Short chain dehydrogenase/reductase family 7C member 2 antibody
- UniProt
- Q96NR8
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