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PKM 抗体 (AA 2-531)

Name: PKM 抗体 (AA 2-531)
Brand: antibodies-online
SKU: ABIN7166916
Availability: OutOfStock

This anti-PKM antibody is a 小鼠单克隆 antibody detecting PKM in ELISA, WB, IHC, IF, IP 和 FACS. Suitable for 人.

Western Blot Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, U251 whole cell lysate, Rat Brain tissue, Rabbit Skeletal Muscle tissue All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1 min (PKM 抗体 (AA 2-531))

IHC image of ABIN7166916 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (PKM 抗体 (AA 2-531))

IHC

Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1 min (PKM 抗体 (AA 2-531))

Western Blot Positive WB detected in: Mouse Heart tissue, Mouse Brain tissue, Mouse Skeletal Muscle tissue All lanes: PKM antibody at 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 55-60 kDa Observed band size: 55-60 kDa (PKM 抗体 (AA 2-531))

Western Blot Positive WB detected in: Rat Heart tissue, Rat Spleen tissue, Rat Brain tissue All lanes: PKM antibody at 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 55-60 kDa Observed band size: 55-60 kDa (PKM 抗体 (AA 2-531))

Western Blot Positive WB detected in: MCF-7 whole cell lysate at 40 μg, 20 μg, 10 μg, 5 μg, 2. (PKM 抗体 (AA 2-531))

Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5 min (PKM 抗体 (AA 2-531))

IHC

9 images

产品编号 ABIN7166916

发货至: 中国

Datasheet as PDF

Quick Overview for PKM 抗体 (AA 2-531) (ABIN7166916)

抗原

See all PKM 抗体

PKM (Pyruvate Kinase M1/2 (PKM))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This PKM antibody is un-conjugated

应用范围

ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

克隆位点

6C3C7

抗原表位
6

5

5

3

2

2

2

2

1

1

1

1

1

1
AA 2-531

交叉反应

人, 小鼠, 兔, 大鼠

纯化方法

Protein G purified

免疫原

Recombinant Human Pyruvate kinase PKM protein (2-531AA)

亚型

IgG1, IgG1 kappa
anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibody
PKM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) antibody
PKM 适用: 人 ELISA, WB, IHC, IF, IP, FACS 宿主: 兔 Monoclonal 7B2 unconjugated Recombinant Antibody

anti-Pyruvate Kinase M1/2 (PKM) (AA 185-461) antibody
PKM 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibody
PKM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (Internal Region) antibody
PKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (AA 2-192) antibody
PKM 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibody
PKM 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) antibody
PKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibody
PKM 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (pTyr105) antibody
PKM 适用: 人, 小鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Recommended dilution: WB: 1:4000-1:256000, IHC: 1:200-1:500, IF: 1:150-1:300, FC: 1:50-1:200, IP: 1 μL-4 μL,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

PKM (Pyruvate Kinase M1/2 (PKM))

别名

PKM

背景

Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Aliases: CTHBP antibody, Cytosolic thyroid hormone-binding protein antibody, KPYM_HUMAN antibody, OIP-3 antibody, Opa-interacting protein 3 antibody, p58 antibody, pkm antibody, PKM1 antibody, PKM2 antibody, Pyruvate kinase 2/3 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase PKM antibody, THBP1 antibody, Thyroid hormone-binding protein 1 antibody, Tumor M2-PK antibody

UniProt

P14618

途径

Warburg Effect