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MEF2A 抗体 (pThr312)

Name: MEF2A 抗体 (pThr312)
Brand: antibodies-online
SKU: ABIN7138832
Availability: OutOfStock

MEF2A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

Western blot analysis of extracts from C2C12 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MEF2A (Phospho-Thr312) Antibody. (MEF2A 抗体 (pThr312))

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEF2A(Phospho-Thr312) Antibody(left) or the same antibody preincubated with blocking peptide(right). (MEF2A 抗体 (pThr312))

IHC

Immunofluorescence staining of methanol-fixed Hela cells using MEF2A(Phospho-Thr312) Antibody. (MEF2A 抗体 (pThr312))

3 images

产品编号 ABIN7138832

发货至: 中国

Datasheet as PDF

抗原 See all MEF2A 抗体

MEF2A (Myocyte Enhancer Factor 2A (MEF2A))
抗原表位
25

22

15

8

5

4

4

4

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr312
适用
126

102

85

10

9

8

8

8

6

6

5

5

4

3

2

2

1
人
宿主
121

7
兔
Compatible Secondaries
克隆类型
111

17
多克隆
标记
66

6

4

4

4

4

4

4

4

4

2

2

2

2

2

2

2

2

2

2

2

1

1
This MEF2A antibody is un-conjugated
应用范围
89

38

36

26

26

25

21

13

6

5

3

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

交叉反应

人, 小鼠, 大鼠

纯化方法

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

免疫原

Peptide sequence around phosphorylation site of threonine 312 (L-A-T(p)-P-V) derived from Human MEF2A.

亚型

IgG

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anti-Myocyte Enhancer Factor 2A (MEF2A) (pThr312) antibody
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MEF2A 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10 unconjugated

anti-Myocyte Enhancer Factor 2A (MEF2A) (pThr319) antibody
MEF2A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Myocyte Enhancer Factor 2A (MEF2A) (C-Term) antibody
MEF2A 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Myocyte Enhancer Factor 2A (MEF2A) (AA 71-170) antibody
MEF2A 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1D11 unconjugated

anti-Myocyte Enhancer Factor 2A (MEF2A) (pThr312) antibody
MEF2A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Myocyte Enhancer Factor 2A (MEF2A) (AA 391-497) antibody
MEF2A 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F9H2 unconjugated

anti-Myocyte Enhancer Factor 2A (MEF2A) (AA 466-507), (C-Term) antibody
MEF2A 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

限制

仅限研究用
状态

Liquid

缓冲液

Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

别名

MEF2A (MEF2A 产品)

背景

Background:
The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain

K Satoh, J Ohnishi, A Sato, et al. (2007) Nemo-Like Kinase-Myocyte Enhancer Factor 2A Signaling Regulates Anterior Formation in Xenopus Development. Molecular and Cellular Biology, 27(21):7623-30.
This article references the use of the #11039 in the following applications :Western blotting
Aliases: ADCAD1 antibody, MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody, MEF2 antibody, MEF2A antibody, MEF2A_HUMAN antibody, Myocyte enhancer factor 2A antibody, Myocyte-specific enhancer factor 2A antibody, RSRFC4 antibody, RSRFC9 antibody, Serum response factor like protein 1 antibody, Serum response factor-like protein 1 antibody

UniProt

Q02078

途径

Neurotrophin Signaling Pathway, Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades