ITGAV/ITGB6 抗体
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北京 101111
Quick Overview for ITGAV/ITGB6 抗体 (ABIN7127264)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- Integrins are heterodimers composed of noncovalently associated transmembrane i± and i² subunits. The 16 i± and 8 i² subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors, both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Mouse fibroblasts retrovirally infected with cDNA to human beta6, Cells expressed a mouse/human avb6 chimera.
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亚型
- IgG2a
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anti-Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6) antibody
ITGAV/ITGB6 适用: 人, 小鼠, 大鼠 FACS, WB, IF (cc), IF (p), IHC (fro), ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA), Immunoprecipitation (1-2 μg/500 μg protein), ,Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL),Optimal dilution for a specific application should be determined.
Positive_Control: Squamous cell carcinoma cells. A375M melanoma cell line. A549 cell line.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))
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别名
- ITGAV & ITGB6
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背景
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Antigen identified by monoclonal antibody L230, CD 51, CD51, Integrin alpha five, integrin alpha V beta 3, Integrin alpha-V light chain, integrin alphaVbeta3, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), ITGAV, MSK 8, Msk8, Vitronectin receptor subunit alpha, VNRA, VTNR, ITGB6, Integrin, beta 6,Integrin alpha V + beta 6
Cellular localisation: Membrane. -
分子量
- 150-160kDa under non-reducing conditions. Under reducing conditions, cleaved into 2 bands of ~125-130kDa +20-25kDa
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基因ID
- 3685, 3694, 436873
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UniProt
- P06756, P18564
抗原
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