Recombinant KRT8, KRT18 抗体
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- 抗原
- KRT8, KRT18
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human CK8 and CK8 proteins
- 亚型
- IgG
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anti-KRT8, KRT18 antibody
适用: 人 IHC, StM 宿主: 小鼠 Monoclonal KRT8-803-KRT18-835 unconjugated
anti-KRT8, KRT18 antibody适用: 人, Cow, 猴, Pig, 绵羊 IHC, StM, FACS, IF, WB 宿主: 小鼠 Monoclonal C-51 unconjugated
anti-KRT8, KRT18 antibody适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rKRT8-18-1346 unconjugated Recombinant Antibody
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- 应用备注
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Known_Application: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7 or A431 cells. Human skin, colon, lung or breast carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- KRT8, KRT18
- 别名
- KRT8 & KRT18
- 背景
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CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18, KRT8/18, CK8/18,Cytokeratin 8/18
Cellular localisation: Cytoplasm. - 分子量
- 52.5kDa (CK8), 45kDa (CK18)
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