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Recombinant Cytokeratin 2 抗体 (AA 300-400)

This anti-Cytokeratin 2 antibody is a 兔 单克隆 antibody detecting Cytokeratin 2 in IHC (f). Suitable for 人.
产品编号 ABIN7127260
发货至: 中国

Quick Overview for Recombinant Cytokeratin 2 抗体 (AA 300-400) (ABIN7127260)

抗原

See all Cytokeratin 2 (KRT76) 抗体
Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This Cytokeratin 2 antibody is un-conjugated

应用范围

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Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
  • 抗原表位

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    AA 300-400

    特异性

    The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 of Type II Cytokeratins

    亚型

    IgG
  • 应用备注

    Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Positive_Control: Skin or squamous cell carcinoma.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

    别名

    KRT76

    背景

    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Type II (Epithelial Marker)
    Cellular localisation: Cytoplasmic

    分子量

    66/65/64/57/62/60/51/54kDa

    基因ID

    51350, 654392

    UniProt

    Q01546
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