KRT5 & KRT6 抗体
This 小鼠 单克隆 antibody specifically detects KRT5 & KRT6 in IHC (f). It exhibits reactivity toward 人.
产品编号 ABIN7127253
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for KRT5 & KRT6 抗体 (ABIN7127253)
抗原
KRT5 & KRT6
适用
人
宿主
小鼠
克隆类型
单克隆
应用范围
Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
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特异性
- It recognizes polypeptides of 58 kDa and 56 kDa, identified as cytokeratin 5 and 6 respectively. It shows no reaction with keratin 1, 8, or 19. It stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma, and to differentiate epitheliod mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody in the identification of mesothelioma and lung squamous cell carcinoma. It does not react with pulmonary adenocarcinoma.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human KRT5 protein
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亚型
- IgG1, IgG2a
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Human prostate or skin tissue.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- KRT5 & KRT6
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背景
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CK5, Keratin-5, KRT5, Cytoskeletal 6A, KRT6A,Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker)
Cellular localisation: Cytoplasmic -
分子量
- 58kDa (KRT5), 56kDa (KRT6A)
抗原
-
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