Recombinant Cytokeratin 2 抗体 (AA 300-400)
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Quick Overview for Recombinant Cytokeratin 2 抗体 (AA 300-400) (ABIN7127252)
抗原
See all Cytokeratin 2 (KRT76) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 300-400
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原理
- Recombinant Cytokeratin, Type II (Epithelial Marker) Antibody
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特异性
- The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.
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交叉反应 (详细)
- Human.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 of Type II Cytokeratins
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亚型
- IgG1, kappa
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Skin or squamous cell carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))
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别名
- KRT76
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背景
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KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
Skin or squamous cell carcinoma.
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分子量
- 66/65/64/57/62/60/51/54kDa
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基因ID
- 51350, 654392
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UniProt
- Q01546
抗原
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