Recombinant Keratin 77 抗体 (AA 100-200)
Quick Overview for Recombinant Keratin 77 抗体 (AA 100-200) (ABIN7127251)
抗原
See all Keratin 77 (KRT77) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 100-200
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特异性
- The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. It usually used in a cytokeratin cocktail with type II cytokeratin antibody.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Synthetic peptide corresponding to Type I Cytokeratins residues within aa100-200 of Type I Cytokeratins
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亚型
- IgG1
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anti-Keratin 77 (KRT77) antibody
KRT77 适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal KRTL-1077 unconjugated
anti-Keratin 77 (KRT77) antibodyKRT77 适用: 人, 大鼠, Cow, 犬, 小鼠, 兔, 小鸡, 猴, Turtle IHC, WB, IF, FACS, StM 宿主: 小鼠 Monoclonal AE-1 unconjugated
anti-Keratin 77 (KRT77) antibodyKRT77 适用: 人 IHC, StM 宿主: 兔 Monoclonal KRTL-1577R unconjugated Recombinant Antibody
anti-Keratin 77 (KRT77) (Internal Region) antibodyKRT77 适用: 人, 大鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Keratin 77 (KRT77) (AA 1-578) antibodyKRT77 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Skin or squamous cell carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Keratin 77 (KRT77)
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别名
- KRT77
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背景
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K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B,Cytokeratin, Type I (Epithelial Marker)
Cellular localisation: Cytoplasmic -
分子量
- 60/53/51/45/40kDa
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基因ID
- 374454, 334989
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UniProt
- Q7Z794
抗原
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