KRT10, KRT13 抗体
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北京 101111
Quick Overview for KRT10, KRT13 抗体 (ABIN7127244)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Cytokeratin 10/13 Antibody
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特异性
- This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
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交叉反应 （详细）
- Human and Cat.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Cytoskeletal preparation extracted from human ectocervical epithelium
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亚型
- IgG2a, kappa
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anti-KRT10, KRT13 antibody
适用: Cat, 人 FACS, IF, IHC, WB 宿主: 小鼠 Monoclonal DE-K13 unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: A431, HeLa or MCF7 cells. Human tonsil, esophageal or bladder carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- KRT10, KRT13
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别名
- KRT10 & KRT13
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背景
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BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT13, Type I keratin Ka13
A431, esophageal or bladder carcinoma., HeLa or MCF7 cells. Human tonsil
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分子量
- 56.5kDa (CK10) & 53kDa (CK13)
抗原
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