MED21 抗体
Quick Overview for MED21 抗体 (ABIN7127181)
抗原
See all MED21 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors. Med6 and Med7, as well as other components of SMCC, associate with co-activator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation. Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human MED21 protein
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亚型
- IgG1
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anti-Mediator Complex Subunit 21 (MED21) (AA 1-144) antibody
MED21 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3E9 unconjugated
anti-Mediator Complex Subunit 21 (MED21) (AA 1-144) antibodyMED21 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4B5 unconjugated
anti-Mediator Complex Subunit 21 (MED21) (AA 1-144) antibodyMED21 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6B6 unconjugated
anti-Mediator Complex Subunit 21 (MED21) antibodyMED21 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 21 (MED21) (AA 1-144) antibodyMED21 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Mediator Complex Subunit 21 (MED21) (AA 19-68) antibodyMED21 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, Pig, 小鸡, Hamster, 猴, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 21 (MED21) (AA 74-123) antibodyMED21 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 斑马鱼, Pig, 小鸡, Hamster, 猴, 兔, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 21 (MED21) (AA 1-144) antibodyMED21 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 21 (MED21) (N-Term) antibodyMED21 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 斑马鱼 WB, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or K562 cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MED21 (Mediator Complex Subunit 21 (MED21))
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别名
- MED21
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背景
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Forkhead box protein Q1, MED21, Hepatocyte nuclear factor 3 forkhead homolog 1, HFH1, HNF3/forkhead like protein 1, MGC77469, Q1, Winged helix / forkhead transcription factor, zgc:77469,MED21 / SRB7
Cellular localisation: Nucleus. Nucleoplasm. Cytoplasm. -
分子量
- 15.5kDa
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基因ID
- 9412, 286145
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UniProt
- Q13503
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途径
- Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
抗原
-
