Recombinant EPX 抗体
Quick Overview for Recombinant EPX 抗体 (ABIN7127088)
抗原
See all EPX 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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特异性
- Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Human eosinophils from a patient with hypereosinophilic syndrome
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亚型
- IgG
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anti-Eosinophil Peroxidase (EPX) (AA 140-300) antibody
EPX 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eosinophil Peroxidase (EPX) (AA 1-715) antibodyEPX 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eosinophil Peroxidase (EPX) (AA 400-500) antibodyEPX 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eosinophil Peroxidase (EPX) (Middle Region) antibodyEPX 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Eosinophil Peroxidase (EPX) (Middle Region) antibodyEPX 适用: 人, 小鼠, 大鼠, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7 cells. Tonsil
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- EPX (Eosinophil Peroxidase (EPX))
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别名
- EPX
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背景
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Eosinophil peroxidase heavy chain, EPER, EPO, EPP, EPX, EPX PEN, PERE,Eosinophil Peroxidase (EPX)
Cellular localisation: Cytoplasmic, granular -
分子量
- 70kDa (55kDa-Heavy Chain, 15kDa-Light Chain)
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基因ID
- 8288, 279259
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UniProt
- P11678
抗原
-
