Vinculin 抗体
Quick Overview for Vinculin 抗体 (ABIN7127045)
抗原
See all Vinculin (VCL) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognizes both Vinculin (125 kDa) and meta-Vinculin (135 kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and i±-Actinin. In addition, vinculin, Talin and i±-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. i±-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
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交叉反应 （详细）
- Human, Mouse, Rat, Cow, Pig, Rabbit, Frog, Fish and Bird.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human Vinculin protein (VCL)
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亚型
- IgG1
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anti-Vinculin (VCL) (AA 1-1066) antibody
VCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C6-1B5 unconjugated
anti-Vinculin (VCL) (AA 879-1064) antibodyVCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) (AA 903-937) antibodyVCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55983 unconjugated
anti-Vinculin (VCL) (AA 1020-1134) antibodyVCL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) (AA 634-668) antibodyVCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55982 unconjugated
anti-Vinculin (VCL) (AA 1-1066) antibodyVCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3F8-1D4 unconjugated
anti-Vinculin (VCL) (AA 174-322) antibodyVCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2575 unconjugated
anti-Vinculin (VCL) (AA 174-322) antibodyVCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2572 unconjugated
anti-Vinculin (VCL) (AA 767-1066) antibodyVCL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) antibodyVCL 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
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应用备注
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Known_Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: A431, K562, NIH3T3, U-87, THP-1 cells. Bladder and Testis
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Vinculin (VCL)
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别名
- VCL
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背景
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CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, VCL, VINC,Vinculin (Marker of Age-related Macular Degeneration)
Cellular localisation: Cytoplasmic -
分子量
- 125-135kDa
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基因ID
- 7414, 643896
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UniProt
- P18206
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途径
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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