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UBE2B 抗体

This anti-UBE2B antibody is a 小鼠 单克隆 antibody detecting UBE2B in IF 和 FACS. Suitable for 人.
产品编号 ABIN7127038
发货至: 中国

Quick Overview for UBE2B 抗体 (ABIN7127038)

抗原

See all UBE2B 抗体
UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

适用

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宿主

  • 27
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小鼠

克隆类型

  • 28
  • 5
单克隆

标记

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This UBE2B antibody is un-conjugated

应用范围

  • 25
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Immunofluorescence (IF), Flow Cytometry (FACS)
  • 特异性

    The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

    交叉反应 (详细)

    Human. Predicted to work in Mouse, Rat, Xenopus and Zebrafish.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant full-length human UBE2B protein

    亚型

    IgG2b
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa, K562 or Jurkat cells.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

    别名

    UBE2B

    物质类

    Viral Protein

    背景

    E2 17 kDa, hHR6B, HR6B, RAD6 homolog B (RAD6B), UBC2, UBE2B, Ubiquitin carrier protein B, Ubiquitin conjugating enzyme E2B, Ubiquitin protein ligase B, Ubiquitin-conjugating enzyme E2 17 kDa,UBE2B
    Cellular localisation: Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.

    分子量

    17kDa

    基因ID

    7320

    UniProt

    P63146
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